| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:100-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | STX1A |
| Gene ID: | 6804 |
| Uniprot ID: | STX1A_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human STX1A (Q16623). |
| Immunogen Sequence: | MKDRTQELRTAKDSDDDDDV AVTVDRDRFMDEFFEQVEEI RGFIDKIAENVEEVKRKHSA ILASPNPDEKTKEELEELMS DIKKTANKVRSKLKSIEQSI |
| Tissue Specificity | Isoform 1: Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2: Expressed in heart, liver, fat, skeletal muscle, kidney and brain. |
| Post Translational Modifications | Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1. Sumoylated, sumoylation is required for regulation of synaptic vesicle endocytosis. |
| Function | Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1). |
| Protein Name | Syntaxin-1a Neuron-Specific Antigen Hpc-1 |
| Database Links | Reactome: R-HSA-181429 Reactome: R-HSA-181430 Reactome: R-HSA-210500 Reactome: R-HSA-212676 Reactome: R-HSA-264642 Reactome: R-HSA-264876 Reactome: R-HSA-422356 Reactome: R-HSA-449836 Reactome: R-HSA-5250971 Reactome: R-HSA-5682910 Reactome: R-HSA-6794361 Reactome: R-HSA-888590 Reactome: R-HSA-9609523 Reactome: R-HSA-9662360 |
| Cellular Localisation | Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle Membrane Single-Pass Type Iv Membrane Protein Synapse Synaptosome Cell Membrane Colocalizes With Kcnb1 At The Cell Membrane Isoform 2: Secreted |
| Alternative Antibody Names | Anti-Syntaxin-1a antibody Anti-Neuron-Specific Antigen Hpc-1 antibody Anti-STX1A antibody Anti-STX1 antibody |
Information sourced from Uniprot.org