Anti-STX17 antibody (1-130) (STJ11102869)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102869
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General Information

Short DescriptionRabbit polyclonal STX17 (1-130) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSTX17
Gene ID55014
Uniprot IDSTX17_HUMAN
Immunogen Region1-130
Immunogen SequenceMSEDEEKVKLRRLEPAIQKF IKIVIPTDLERLRKHQINIE KYQRCRIWDKLHEEHINAGR TVQQLRSNIREIEKLCLKVR KDDLVLLKRMIDPVKEEASA ATAEFLQLHLESVEELKKQF NDEETLLQPP
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human STX17 (NP_060389.2).

Additional Info

Post Translational Modifications Phosphorylated at Tyr-157 probably by ABL1. Dephosphorylation by PTPN2.regulates exit from the endoplasmic reticulum. (Microbial infection) Cleaved by the L.pneumophila serine protease Lpg1137, impairing endoplasmic reticulum-mitochondria communication, leading to inhibit autophagy.
Function SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.
Protein Name Syntaxin-17
Database Links Reactome: R-HSA-204005
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Smooth Endoplasmic Reticulum Membrane
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Cytoplasmic Vesicle
Autophagosome Membrane
Copii-Coated Vesicle Membrane
Cytoplasm
Cytosol
Mitochondrion Membrane
Autolysosome Membrane
Has A Hairpin-Like Insertion Into Membranes
Localizes To The Completed Autophagosome Membrane Upon Cell Starvation
Colocalized With Rab39a And Rab39b In Autolysosomes In Autophagy-Induced Conditions
Alternative Antibody Names Anti-Syntaxin-17 antibody
Anti-STX17 antibody

Information sourced from Uniprot.org

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