• Western blot analysis of mouse heart, using STX17 antibody (STJ11102869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HeLa cells using STX17 rabbit polyclonal antibody (STJ11102869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using STX17 rabbit polyclonal antibody (STJ11102869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-STX17 antibody (1-130) (STJ11102869)

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STJ11102869

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STX17 (1-130) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX17
Gene ID: 55014
Uniprot ID: STX17_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human STX17 (NP_060389.2).
Immunogen Sequence: MSEDEEKVKLRRLEPAIQKF IKIVIPTDLERLRKHQINIE KYQRCRIWDKLHEEHINAGR TVQQLRSNIREIEKLCLKVR KDDLVLLKRMIDPVKEEASA ATAEFLQLHLESVEELKKQF NDEETLLQPP
Post Translational Modifications Phosphorylated at Tyr-157 probably by ABL1. Dephosphorylation by PTPN2.regulates exit from the endoplasmic reticulum. (Microbial infection) Cleaved by the L.pneumophila serine protease Lpg1137, impairing endoplasmic reticulum-mitochondria communication, leading to inhibit autophagy.
Function SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. May also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.
Protein Name Syntaxin-17
Database Links Reactome: R-HSA-204005
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Smooth Endoplasmic Reticulum Membrane
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Cytoplasmic Vesicle
Autophagosome Membrane
Copii-Coated Vesicle Membrane
Cytoplasm
Cytosol
Mitochondrion Membrane
Has A Hairpin-Like Insertion Into Membranes
Localizes To The Completed Autophagosome Membrane Upon Cell Starvation
Alternative Antibody Names Anti-Syntaxin-17 antibody
Anti-STX17 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance