• Immunofluorescence analysis of U-2 OS cells using STIM2 Rabbit polyclonal antibody (STJ119784) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using STIM2 Rabbit polyclonal antibody (STJ119784) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of STIM2 in paraffin-embedded Mouse brain using STIM2 Rabbit polyclonal antibody (STJ119784) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of STIM2 in paraffin-embedded Rat brain using STIM2 Rabbit polyclonal antibody (STJ119784) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from 293T cells, using STIM2 Rabbit polyclonal antibody (STJ119784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-STIM2 antibody (60-180) (STJ119784)

SKU:
STJ119784

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STIM2 (60-180) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STIM2
Gene ID: 57620
Uniprot ID: STIM2_HUMAN
Immunogen Region: 60-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human STIM2 (NP_001162588).
Immunogen Sequence: CFTEEDRFSLEALQTIHKQM DDDKDGGIEVEESDEFIRED MKYKDATNKHSHLHREDKHI TIEDLWKRWKTSEVHNWTLE DTLQWLIEFVELPQYEKNFR DNNVKGTTLPRIAVHEPSFM I
Tissue Specificity Expressed in all tissues and tumor cell lines examined.
Post Translational Modifications Glycosylated. Phosphorylated predominantly on Ser residues.
Function Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
Protein Name Stromal Interaction Molecule 2
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Dynamically Translocates From A Uniform Endoplasmic Reticulum Distribution To Punctual Endoplasmic Reticulum-Plasma Membrane Junctions In Response To Decrease In Endoplasmic Reticulum Ca(2+) Concentration
Alternative Antibody Names Anti-Stromal Interaction Molecule 2 antibody
Anti-STIM2 antibody
Anti-KIAA1482 antibody

Information sourced from Uniprot.org

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