Anti-GNAO1 antibody (60-180) (STJ23814)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GNAO1 (60-180) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GNAO1 |
| Gene ID | 2775 |
| Uniprot ID | GNAO_HUMAN |
| Immunogen Region | 60-180 |
| Immunogen Sequence | GFSGEDVKQYKPVVYSNTIQ SLAAIVRAMDTLGIEYGDKE RKADAKMVCDVVSRMEDTEP FSAELLSAMMRLWGDSGIQE CFNRSREYQLNDSAKYYLDS LDRIGAADYQPTEQDILRTR V |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human GNAO1 (NP_066268.1). |
Additional Info
| Post Translational Modifications | Histaminylated at Gln-205 residues by TGM2. Palmitoylated at Cys-351, leading to binding to ADGRG3. |
| Function | Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. |
| Protein Name | Guanine Nucleotide-Binding Protein G(O Subunit Alpha |
| Database Links | Reactome: R-HSA-4086398 |
| Cellular Localisation | Cell MembraneMembraneLipid-Anchor |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein G(O Subunit Alpha antibodyAnti-GNAO1 antibody |
Information sourced from Uniprot.org