• Western blot analysis of extracts of various cell lines, using GNAO1 antibody (STJ23814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Immunofluorescence analysis of mouse brain cells using GNAO1 rabbit polyclonal antibody (STJ23814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain cells using GNAO1 rabbit polyclonal antibody (STJ23814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GNAO1 antibody (60-180) (STJ23814)

SKU:
STJ23814

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GNAO1 (60-180) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNAO1
Gene ID: 2775
Uniprot ID: GNAO_HUMAN
Immunogen Region: 60-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human GNAO1 (NP_066268.1).
Immunogen Sequence: GFSGEDVKQYKPVVYSNTIQ SLAAIVRAMDTLGIEYGDKE RKADAKMVCDVVSRMEDTEP FSAELLSAMMRLWGDSGIQE CFNRSREYQLNDSAKYYLDS LDRIGAADYQPTEQDILRTR V
Post Translational Modifications Histaminylated at Gln-205 residues by TGM2.
Function Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14.
Protein Name Guanine Nucleotide-Binding Protein G(O Subunit Alpha
Database Links Reactome: R-HSA-4086398
Cellular Localisation Cell Membrane
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein G(O Subunit Alpha antibody
Anti-GNAO1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance