• Western blot analysis of extracts of various cell lines, using STIM1 antibody (STJ11100938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HeLa cells using STIM1 rabbit polyclonal antibody (STJ11100938) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using STIM1 rabbit polyclonal antibody (STJ11100938) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of K-562 cells using STIM1 rabbit polyclonal antibody (STJ11100938) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g STIM1 antibody (STJ11100938). Western blot was performed from the immunoprecipitate using STIM1 (STJ11100938) at a dilution of 1:1000.

Anti-STIM1 antibody (530-685) (STJ11100938)

SKU:
STJ11100938

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STIM1 (530-685) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STIM1
Gene ID: 6786
Uniprot ID: STIM1_HUMAN
Immunogen Region: 530-685
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 530-685 of human STIM1 (NP_003147.2).
Immunogen Sequence: RQRVAPKPPQMSRAADEALN AMTSNGSHRLIEGVHPGSLV EKLPDSPALAKKALLALNHG LDKAHSLMELSPSAPPGGSP HLDSSRSHSPSSPDPDTPSP VGDSRALQASRNTRIPHLAG KKAVAEEDNGSIGEETDSSP GRKKFPLKIFKKPLKK
Tissue Specificity Ubiquitously expressed in various human primary cells and tumor cell lines.
Post Translational Modifications Glycosylation is required for cell surface expression. Phosphorylated predominantly on Ser residues.
Function Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1. Involved in enamel formation. Activated following interaction with STIMATE, leading to promote STIM1 conformational switch.
Protein Name Stromal Interaction Molecule 1
Database Links Reactome: R-HSA-139853
Reactome: R-HSA-5578775
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum Membrane
Cytoplasm
Cytoskeleton
Sarcoplasmic Reticulum
Translocates From The Endoplasmic Reticulum To The Cell Membrane In Response To A Depletion Of Intracellular Calcium And Is Detected At Punctae Corresponding To Junctions Between The Endoplasmic Reticulum And The Cell Membrane
Associated With The Microtubule Network At The Growing Distal Tip Of Microtubules
Colocalizes With Orai1 At The Cell Membrane
Colocalizes Preferentially With Casq1 At Endoplasmic Reticulum In Response To A Depletion Of Intracellular Calcium
Alternative Antibody Names Anti-Stromal Interaction Molecule 1 antibody
Anti-STIM1 antibody
Anti-GOK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance