| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-STAT1 is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
SM5222 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB (H, M) 1:500-1:1000 |
| Storage Instruction: |
Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
| Gene Symbol: |
Stat1 |
| Uniprot ID: |
STAT1_MOUSE |
| Immunogen: |
KLH conjugated Synthetic peptide corresponding to Mouse STAT1 |
| Function | Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-748 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6. Phosphorylation at Thr-748 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription. Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine. |
| Protein Name | Signal Transducer And Activator Of Transcription 1 |
| Cellular Localisation | CytoplasmNucleusTranslocated Into The Nucleus Upon Tyrosine Phosphorylation And DimerizationIn Response To Ifn-Gamma And Signaling By Activated Fgfr1Fgfr2Fgfr3 Or Fgfr4Monomethylation At Lys-525 Is Required For Phosphorylation At Tyr-701 And Translocation Into The NucleusTranslocates Into The Nucleus In Response To Interferon-Beta Stimulation |
| Alternative Antibody Names | Anti-Signal Transducer And Activator Of Transcription 1 antibodyAnti-Stat1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
