• Western blot analysis of A-431, using SRF antibody (STJ11100944) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates, using SRF antibody (STJ11100944) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SRF rabbit polyclonal antibody (STJ11100944) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal using SRF rabbit polyclonal antibody (STJ11100944) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using SRF rabbit polyclonal antibody (STJ11100944) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SRF antibody (321-420) (STJ11100944)

SKU:
STJ11100944

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRF
Gene ID: 6722
Uniprot ID: SRF_HUMAN
Immunogen Region: 321-420
Specificity: A synthetic peptide corresponding to a sequence within amino acids 321-420 of human SRF (NP_003122.1).
Immunogen Sequence: TVLKSTGSGPVSSGGLMQLP TSFTLMPGGAVAQQVPVQAI QVHQAPQQASPSRDSSTDLT QTSSSGTVTLPATIMTSSVP TTVGGHMMYPSPHAVMYAPT
Post Translational Modifications Phosphorylated by PRKDC.
Function SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Protein Name Serum Response Factor
Srf
Database Links Reactome: R-HSA-5663220
Reactome: R-HSA-9031628
Reactome: R-HSA-9634638
Reactome: R-HSA-9733709
Reactome: R-HSA-9768777
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Serum Response Factor antibody
Anti-Srf antibody
Anti-SRF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance