• Western blot analysis of HepG2, using SQLE antibody (STJ111151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of HeLa cells using SQLE rabbit polyclonal antibody (STJ111151) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using SQLE rabbit polyclonal antibody (STJ111151) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using SQLE rabbit polyclonal antibody (STJ111151) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using SQLE rabbit polyclonal antibody (STJ111151) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-SQLE antibody (300-574) (STJ111151)

SKU:
STJ111151

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SQLE (300-574) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SQLE
Gene ID: 6713
Uniprot ID: ERG1_HUMAN
Immunogen Region: 300-574
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-574 of human SQLE (NP_003120.2).
Immunogen Sequence: VSVSSHFVGFLMKNAPQFKA NHAELILANPSPVLIYQISS SETRVLVDIRGEMPRNLREY MVEKIYPQIPDHLKEPFLEA TDNSHLRSMPASFLPPSSVK K
Tissue Specificity Detected in liver (at protein level).
Post Translational Modifications Ubiquitinated by MARCHF6 in response to high cholesterol levels in intracellular membranes, leading to proteasomal degradation.
Function Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.
Protein Name Squalene Monooxygenase
Squalene Epoxidase
Se
Database Links Reactome: R-HSA-191273
Reactome: R-HSA-2426168
Cellular Localisation Microsome Membrane
Peripheral Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Squalene Monooxygenase antibody
Anti-Squalene Epoxidase antibody
Anti-Se antibody
Anti-SQLE antibody
Anti-ERG1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance