| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPRY2 |
| Gene ID: | 10253 |
| Uniprot ID: | SPY2_HUMAN |
| Immunogen Region: | 216-315 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 216-315 of human Sprouty 2 (O43597). |
| Immunogen Sequence: | GTCVCCVKGLFYHCSNDDED NCADNPCSCSQSHCCTRWSA MGVMSLFLPCLWCYLPAKGC LKLCQGCYDRVNRPGCRCKN SNTVCCKVPTVPPRNFEKPT |
| Post Translational Modifications | Cleaved at Pro-144 by the prolyl endopeptidase FAP (seprase) activity (in vitro). |
| Function | Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2. Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis. Inhibits TGFB-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells. Inhibits CBL/C-CBL-mediated EGFR ubiquitination. |
| Protein Name | Protein Sprouty Homolog 2Spry-2 |
| Database Links | Reactome: R-HSA-1295596Reactome: R-HSA-182971 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionRuffle MembraneAssociated With Microtubules In Unstimulated Cells But Is Translocated To The Membrane Ruffles In Cells Stimulated With Egf (Epidermal Growth Factor) |
| Alternative Antibody Names | Anti-Protein Sprouty Homolog 2 antibodyAnti-Spry-2 antibodyAnti-SPRY2 antibody |
Information sourced from Uniprot.org