| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SPP1 (17-314) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SPP1 |
| Gene ID: |
6696 |
| Uniprot ID: |
OSTP_HUMAN |
| Immunogen Region: |
17-314 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human Osteopontin (NP_001035147.1). |
| Immunogen Sequence: |
IPVKQADSGSSEEKQLYNKY PDAVATWLNPDPSQKQNLLA PQNAVSSEETNDFKQETLPS KSNESHDHMDDMDDEDDDDH VDSQDSIDSNDSDDVDDTDD SHQSDESHHSDESDELVTDF PTDLPATEVFTPVVPTVDTY DGRGDSVVYGLRSKSKKFRR PDIQYPDATDEDITSHMESE ELNGAYKAIPVAQDLNAPSD WDSRGKDSYETSQLDDQSAE THSHKQSRLYKRKANDESN |
| Tissue Specificity | Detected in cerebrospinal fluid and urine (at protein level). Bone. Found in plasma. |
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | OsteopontinBone Sialoprotein 1NephropontinSecreted Phosphoprotein 1Spp-1Urinary Stone ProteinUropontin |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-186797Reactome: R-HSA-216083Reactome: R-HSA-381426Reactome: R-HSA-8949275Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Osteopontin antibodyAnti-Bone Sialoprotein 1 antibodyAnti-Nephropontin antibodyAnti-Secreted Phosphoprotein 1 antibodyAnti-Spp-1 antibodyAnti-Urinary Stone Protein antibodyAnti-Uropontin antibodyAnti-SPP1 antibodyAnti-BNSP antibodyAnti-OPN antibodyAnti-PSEC0156 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
