| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPP1 |
| Gene ID: | 6696 |
| Uniprot ID: | OSTP_HUMAN |
| Immunogen Region: | 17-314 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human Osteopontin (NP_001035147.1). |
| Immunogen Sequence: | IPVKQADSGSSEEKQLYNKY PDAVATWLNPDPSQKQNLLA PQNAVSSEETNDFKQETLPS KSNESHDHMDDMDDEDDDDH VDSQDSIDSNDSDDVDDTDD SHQSDESHHSDESDELVTDF PTDLPATEVFTPVVPTVDTY DGRGDSVVYGLRSKSKKFRR PDIQYPDATDEDITSHMESE ELNGAYKAIPVAQDLNAPSD WDSRGKDSYETSQLDDQSAE THSHKQSRLYKRKANDESN |
| Tissue Specificity | Detected in cerebrospinal fluid and urine (at protein level). Bone. Found in plasma. |
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | Osteopontin Bone Sialoprotein 1 Nephropontin Secreted Phosphoprotein 1 Spp-1 Urinary Stone Protein Uropontin |
| Database Links | Reactome: R-HSA-1474228 Reactome: R-HSA-186797 Reactome: R-HSA-216083 Reactome: R-HSA-381426 Reactome: R-HSA-8949275 Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Osteopontin antibody Anti-Bone Sialoprotein 1 antibody Anti-Nephropontin antibody Anti-Secreted Phosphoprotein 1 antibody Anti-Spp-1 antibody Anti-Urinary Stone Protein antibody Anti-Uropontin antibody Anti-SPP1 antibody Anti-BNSP antibody Anti-OPN antibody Anti-PSEC0156 antibody |
Information sourced from Uniprot.org
