Anti-SPIB antibody (1-262) (STJ29587)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ29587
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General Information

Short DescriptionRabbit polyclonal SPIB (1-262) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSPIB
Gene ID6689
Uniprot IDSPIB_HUMAN
Immunogen Region1-262
Immunogen SequenceMLALEAAQLDGPHFSCLYPD GVFYDLDSCKHSSYPDSEGA PDSLWDWTVAPPVPATPYEA FDPAAAAFSHPQAAQLCYEP PTYSPAGNLELAPSLEAPGP GLPAYPTENFASQTLVPPAY APYPSPVLSEEEDLPLDSPA LEVSDSESDEALVAGPEGKG SEAGTRKKLRLYQFLLGLLT RGDMRECVWWVEPGAGVFQF SSKHKELLARRWGQQKGNRK RMTYQKLARALRNYAKTGE
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIB (NP_003112.2).

Additional Info

Tissue Specificity Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.
Function Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
Protein Name Transcription Factor Spi-B
Database Links
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Transcription Factor Spi-B antibody
Anti-SPIB antibody

Information sourced from Uniprot.org

Citations

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