Anti-Spectrin, alpha II antibody (676-2, 400) (STJA0040968)

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STJA0040968-100
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Host: Rabbit
Applications: ICC/WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Rabbit polyclonal anti-Spectrin, alpha II (676-2, 400) for use in ICC and WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Lyophilized with sodium azide.
Purification: Whole serum
Dilution Range: WB 1:5000-1:10000
ICC 1:500-1:1000
Storage Instruction: Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at-20°C to
Gene Symbol: SPTAN1
Gene ID: 6709
Uniprot ID: SPTN1_HUMAN
Immunogen Region: 676-2, 400
Specificity: The antibody reacts with a 240 kDa band by Western blot on mouse sciatic nerve extract. Minor bands below may be seen and this likely represents in vivo proteolytic fragments of alpha-II spectrin. It has also been used successfully for immunocytochem
Immunogen: The antibody was raised against a mix of five recombinant constructs containing the entire C-terminal region of human alpha-II spectrin (amino acids 676-2, 400).
Immunogen Sequence: Human
Post Translational Modifications Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Function Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Protein Name Spectrin Alpha Chain - Non-Erythrocytic 1
Alpha-Ii Spectrin
Fodrin Alpha Chain
Spectrin - Non-Erythroid Alpha Subunit
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-373753
Reactome: R-HSA-375165
Reactome: R-HSA-445095
Reactome: R-HSA-5673001
Reactome: R-HSA-6798695
Reactome: R-HSA-6807878
Reactome: R-HSA-9013420
Reactome: R-HSA-9013424
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Expressed Along The Cell Membrane In Podocytes And Presumptive Tubule Cells During Glomerulogenesis And Is Expressed Along Lateral Cell Margins In Tubule Cells
Alternative Antibody Names Anti-Spectrin Alpha Chain - Non-Erythrocytic 1 antibody
Anti-Alpha-Ii Spectrin antibody
Anti-Fodrin Alpha Chain antibody
Anti-Spectrin - Non-Erythroid Alpha Subunit antibody
Anti-SPTAN1 antibody
Anti-NEAS antibody
Anti-SPTA2 antibody

Information sourced from Uniprot.org