Anti-Spectrin alpha chain, non-erythrocytic 1 antibody (676-1043) [3D7] (STJA0040763)

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STJA0040763-100
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Host: Mouse
Applications: FC/ICC/IHC-F/WB
Reactivity: Bovine/Human/Mouse/Pig/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Mouse monoclonal anti-Spectrin alpha chain, non-erythrocytic 1 (676-1043) for use in FC, ICC, IHC-F and WB in Bovine, Human, Mouse, Pig and Rat samples. Datasheet included with dilution recommendations, and related reagents.
Clonality : Monoclonal
Clone ID : 3D7
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
Purification: Affinity-purified
Dilution Range: WB 1:5000-1:10000
IHC 1:1000-1:2000
ICC 1:1000-1:2000
Storage Instruction: Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Aliquot and store at-20°C for a higher stability and at 2-8°C with an approp
Gene Symbol: SPTAN1
Gene ID: 6709
Uniprot ID: SPTN1_HUMAN
Immunogen Region: 676-1043
Specificity: Human; bovine; porcine; rat;.
Immunogen: This antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (amino acids 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in
Immunogen Sequence: Human
Post Translational Modifications Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
Function Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Protein Name Spectrin Alpha Chain - Non-Erythrocytic 1
Alpha-Ii Spectrin
Fodrin Alpha Chain
Spectrin - Non-Erythroid Alpha Subunit
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-373753
Reactome: R-HSA-375165
Reactome: R-HSA-445095
Reactome: R-HSA-5673001
Reactome: R-HSA-6798695
Reactome: R-HSA-6807878
Reactome: R-HSA-9013420
Reactome: R-HSA-9013424
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Expressed Along The Cell Membrane In Podocytes And Presumptive Tubule Cells During Glomerulogenesis And Is Expressed Along Lateral Cell Margins In Tubule Cells
Alternative Antibody Names Anti-Spectrin Alpha Chain - Non-Erythrocytic 1 antibody
Anti-Alpha-Ii Spectrin antibody
Anti-Fodrin Alpha Chain antibody
Anti-Spectrin - Non-Erythroid Alpha Subunit antibody
Anti-SPTAN1 antibody
Anti-NEAS antibody
Anti-SPTA2 antibody

Information sourced from Uniprot.org