| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPAM1 |
| Gene ID: | 6677 |
| Uniprot ID: | HYALP_HUMAN |
| Immunogen Region: | 100-300 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2). |
| Immunogen Sequence: | PYIDSITGVTVNGGIPQKIS LQDHLDKAKKDITFYMPVDN LGMAVIDWEEWRPTWARNWK PKDVYKNRSIELVQQQNVQL SLTEATEKAKQEFEKAGKDF LVETIKLGKLLRPNHLWGYY LFPDCYNHHYKKPGYNGSCF NVEIKRNDDLSWLWNESTAL YPSIYLNTQQSPVAATLYVR NRVREAIRVSKIPDAKSPLP V |
| Tissue Specificity | Testis. |
| Post Translational Modifications | N-glycosylated. |
| Function | Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. |
| Protein Name | Hyaluronidase Ph-20Hyal-Ph20Hyaluronoglucosaminidase Ph-20Sperm Adhesion Molecule 1Sperm Surface Protein Ph-20 |
| Database Links | Reactome: R-HSA-2534343 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Antibody Names | Anti-Hyaluronidase Ph-20 antibodyAnti-Hyal-Ph20 antibodyAnti-Hyaluronoglucosaminidase Ph-20 antibodyAnti-Sperm Adhesion Molecule 1 antibodyAnti-Sperm Surface Protein Ph-20 antibodyAnti-SPAM1 antibodyAnti-HYAL3 antibodyAnti-PH20 antibody |
Information sourced from Uniprot.org