• Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SPAG8 antibody (STJ192199)

SKU:
STJ192199

€57.50 - €282.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Sperm-associated antigen 8 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPAG8
Gene ID: 26206
Uniprot ID: SPAG8_HUMAN
Specificity: SPAG8 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein
Function Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau. Involved in the acrosome reaction and in binding of sperm to the zona pellucida. Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis. May play a role in fertility and microtubule formation through interaction with RANBP9.
Protein Name Sperm-Associated Antigen 8
Hsd-1
Sperm Membrane Protein 1
Smp-1
Sperm Membrane Protein Bs-84
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Cytoskeleton
Microtubule Organizing Center
Spindle
Cilium Axoneme
In Mature Sperm Cells
Detected In The Acrosomal Region Of The Head And In The Middle Piece Of The Tail
Localized To The Nucleus And Cytoplasm Of Spermatocytes And Round Spermatids While
In Elongating Spermatids
Expressed In The Cytoplasm But Not In The Nucleus
During The Cell Cycle
Localized On The Microtubule-Organizing Center (Mtoc) During Prophase
In Metaphase
Extends Along Spindle Microtubules
In Anaphase
Detected On The Astral Microtubules And Mid-Zone
In Telophase
Remains At The Mid-Zone
After Cytokinesis
Returns To The Mtoc
Microtubule Inner Protein (Mip) Part Of The Dynein-Decorated Doublet Microtubules (Dmts) In Cilia Axoneme
Alternative Antibody Names Anti-Sperm-Associated Antigen 8 antibody
Anti-Hsd-1 antibody
Anti-Sperm Membrane Protein 1 antibody
Anti-Smp-1 antibody
Anti-Sperm Membrane Protein Bs-84 antibody
Anti-SPAG8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance