| Host: | Goat |
| Applications: | Pep-ELISA/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SORBS1/ponsin (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:2000. WB: Preliminary experiments in Human Adipose, Heart, Kidney, Liver and Muscle lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). IHC: Paraffin |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SORBS1 |
| Gene ID: | 10580 |
| Uniprot ID: | SRBS1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1 |
| Specificity: | This antibody is expected to recognise all 7 reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1). |
| Immunogen Sequence: | SPKSEDDDSDLYSP |
| Post Translational Modifications | O-glycosylated. |
| Function | Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions. |
| Protein Name | Sorbin And Sh3 Domain-Containing Protein 1 Ponsin Sh3 Domain Protein 5 Sh3p12 C-Cbl-Associated Protein Cap |
| Database Links | Reactome: R-HSA-445355 |
| Cellular Localisation | Cell Junction Adherens Junction Cell Membrane Cytoplasm Cytoskeleton Focal Adhesion Nucleus Nucleus Matrix Colocalizes With The Ten-1 Icd Form Of Tenm1 In The Nucleus Colocalizes With Actin Stress Fibers Also Detected At The Plasma Membrane And In Neuronal Intranuclear Inclusions Colocalized With Pxn At Focal Adhesions During Myogenic Differentiation |
| Alternative Antibody Names | Anti-Sorbin And Sh3 Domain-Containing Protein 1 antibody Anti-Ponsin antibody Anti-Sh3 Domain Protein 5 antibody Anti-Sh3p12 antibody Anti-C-Cbl-Associated Protein antibody Anti-Cap antibody Anti-SORBS1 antibody Anti-KIAA0894 antibody Anti-KIAA1296 antibody Anti-SH3D5 antibody |
Information sourced from Uniprot.org