• Western blot analysis of extracts of K-562 cells, using SOCS2 rabbit monoclonal antibody (STJ11102450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Confocal imaging of MCF7 cells using SOCS2 Rabbit monoclonal antibody (STJ11102450, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of extracts of various cell lines, using SOCS2 rabbit monoclonal antibody (STJ11102450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Confocal imaging of C2C12 cells using SOCS2 Rabbit monoclonal antibody (STJ11102450, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded rat lung using SOCS2 rabbit monoclonal antibody (STJ11102450) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SOCS2 in paraffin-embedded rat lung using SOCS2 Rabbit monoclonal antibody (STJ11102450) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using SOCS2 rabbit monoclonal antibody (STJ11102450) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SOCS2 Rabbit monoclonal antibody (STJ11102450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunofluorescence analysis of U-2 OS cells using SOCS2 rabbit monoclonal antibody (STJ11102450) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from K-562 cells, using SOCS2 Rabbit monoclonal antibody (STJ11102450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SOCS2 antibody (1-198) [S0MR] (STJ11102450)

SKU:
STJ11102450

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:4000
IHC-P:1:100-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOCS2
Gene ID: 8835
Uniprot ID: SOCS2_HUMAN
Immunogen Region: 1-198
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human SOCS2 (NP_001257400.1)
Immunogen Sequence: MTLRCLEPSGNGGEGTRSQW GTAGSAEEPSPQAARLAKAL RELGQTGWYWGSMTVNEAKE KLKEAPEGTFLIRDSSHSDY LLTISVKTSAGPTNLRIEYQ DGKFRLDSIICVKSKLKQFD SVVHLIDYYVQMCKDKRTGP EAPRNGTVHLYLTKPLYTSA PSLQHLCRLTINKCTGAIWG LPLPTRLKDYLEEYKFQV
Tissue Specificity High expression in heart, placenta, lung, kidney and prostate. Predominantly expressed in pulmonary epithelia cells, specifically type II pneumocytes.
Post Translational Modifications Ubiquitinated.mediated by AREL1 and leading to its subsequent proteasomal degradation. Ubiquitination is dependent on its phosphorylation at Ser-52, by PKC. Ubiquitination is stimulated by LPS. Phosphorylation at Ser-52 by PKC facilitates its ubiquitination and proteasomal degradation.
Function Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as EPOR and GHR. Specifically recognizes and binds phosphorylated proteins via its SH2 domain, promoting their ubiquitination. The ECS(SOCS2) complex acts as a key regulator of growth hormone receptor (GHR) levels by mediating ubiquitination and degradation of GHR, following GHR phosphorylation by JAK2. The ECS(SOCS2) also catalyzes ubiquitination and degradation of JAK2-phosphorylated EPOR.
Protein Name Suppressor Of Cytokine Signaling 2
Socs-2
Cytokine-Inducible Sh2 Protein 2
Cis-2
Stat-Induced Stat Inhibitor 2
Ssi-2
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-8951664
Reactome: R-HSA-9706369
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Suppressor Of Cytokine Signaling 2 antibody
Anti-Socs-2 antibody
Anti-Cytokine-Inducible Sh2 Protein 2 antibody
Anti-Cis-2 antibody
Anti-Stat-Induced Stat Inhibitor 2 antibody
Anti-Ssi-2 antibody
Anti-SOCS2 antibody
Anti-CIS2 antibody
Anti-SSI2 antibody
Anti-STATI2 antibody

Information sourced from Uniprot.org

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