• Western blot analysis of extracts of various cell lines, using SNX15 antibody (STJ116345) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of L929 cells using SNX15 Rabbit polyclonal antibody (STJ116345) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SNX15 antibody (STJ116345) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SNX15 Rabbit polyclonal antibody (STJ116345) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SNX15 antibody (STJ116345) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SNX15 Rabbit polyclonal antibody (STJ116345) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using SNX15 antibody (STJ116345) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SNX15 Rabbit polyclonal antibody (STJ116345) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SNX15 antibody (1-342) (STJ116345)

SKU:
STJ116345

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNX15 (1-342) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNX15
Gene ID: 29907
Uniprot ID: SNX15_HUMAN
Immunogen Region: 1-342
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human SNX15 (NP_037438.2).
Immunogen Sequence: MSRQAKDDFLRHYTVSDPRT HPKGYTEYKVTAQFISKKDP EDVKEVVVWKRYSDFRKLHG DLAYTHRNLFRRLEEFPAFP RAQVFGRFEASVIEERRKGA EDLLRFTVHIPALNNSPQLK EFFRGGEVTRPLEVSRDLHI LPPPLIPTPPPDDPRLSQLL PAERRGLEELEVPVDPPPSS PAQEALDLLFNCESTEEASG SPARGPLTEAELALFDPFSK EEGAAPSPTHVAELATMEV
Tissue Specificity Widely expressed.
Function May be involved in several stages of intracellular trafficking. Overexpression of SNX15 disrupts the normal trafficking of proteins from the plasma membrane to recycling endosomes or the TGN.
Protein Name Sorting Nexin-15
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle Membrane
Alternative Antibody Names Anti-Sorting Nexin-15 antibody
Anti-SNX15 antibody

Information sourced from Uniprot.org

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