• Immunoprecipitation of U1A/SNRPA from 300 µg extracts of HeLa cells was performed using 3 µg of U1A/SNRPA Rabbit monoclonal antibody (STJ11101925). Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using U1A/SNRPA Rabbit monoclonal antibody (STJ11101925) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using U1A/SNRPA Rabbit monoclonal antibody (STJ11101925) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using U1A/SNRPA Rabbit monoclonal antibody (STJ11101925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SNRPA antibody (1-100) [S5MR] (STJ11101925)

SKU:
STJ11101925

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-U1A (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNRPA
Gene ID: 6626
Uniprot ID: SNRPA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human U1A/SNRPA (P09012).
Immunogen Sequence: MAVPETRPNHTIYINNLNEK IKKDELKKSLYAIFSQFGQI LDILVSRSLKMRGQAFVIFK EVSSATNALRSMQGFPFYDK PMRIQYAKTDSDIIAKMKGT
Function Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
Protein Name U1 Small Nuclear Ribonucleoprotein A
U1 Snrnp A
U1-A
U1a
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-U1 Small Nuclear Ribonucleoprotein A antibody
Anti-U1 Snrnp A antibody
Anti-U1-A antibody
Anti-U1a antibody
Anti-SNRPA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance