• Immunofluorescence analysis of U-2 OS cells using SNIP1 Rabbit polyclonal antibody (STJ119155) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SNIP1 in paraffin-embedded human colon carcinoma using SNIP1 Rabbit polyclonal antibody (STJ119155) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from BxPC-3 cells, using SNIP1 Rabbit polyclonal antibody (STJ119155) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SNIP1 antibody (200-300) (STJ119155)

SKU:
STJ119155

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNIP1 (200-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNIP1
Gene ID: 79753
Uniprot ID: SNIP1_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SNIP1 (NP_078976.2).
Immunogen Sequence: SESQELVPRPGGNNKEKEVP AKEKPSFELSGALLEDTNTF RGVVIKYSEPPEARIPKKRW RLYPFKNDEVLPVMYIHRQS AYLLGRHRRIADIPIDHPSC S
Tissue Specificity Ubiquitous, with highest expression in heart and skeletal muscle.
Post Translational Modifications Degraded by the proteasome upon binding to the SMAD1/OAZ1/PSMB4 complex.
Function Required for pre-mRNA splicing as component of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
Protein Name Smad Nuclear-Interacting Protein 1
Fha Domain-Containing Protein Snip1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Smad Nuclear-Interacting Protein 1 antibody
Anti-Fha Domain-Containing Protein Snip1 antibody
Anti-SNIP1 antibody

Information sourced from Uniprot.org

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