Anti-SNAP25 antibody (193-206 aa) [R07-1D7] (STJA0013092)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human SNAP25
General Information
| Short Description | Rabbit monoclonal anti-SNAP25 (193-206 aa) for use in WB, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R07-1D7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SNAP25 |
| Gene ID | 6616 |
| Uniprot ID | SNP25_HUMAN |
| Immunogen | A synthetic peptide of human SNAP25 |
| Immunogen Region | 193-206 aa |
Additional Info
| Tissue Specificity | Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. |
| Post Translational Modifications | Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release. C.botulinum type C only rarely infects humans. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release. |
| Function | t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. |
| Protein Name | Synaptosomal-Associated Protein 25Snap-25Super ProteinSupSynaptosomal-Associated 25 Kda Protein |
| Database Links | Reactome: R-HSA-181429Reactome: R-HSA-181430Reactome: R-HSA-210500Reactome: R-HSA-212676Reactome: R-HSA-264642Reactome: R-HSA-422356Reactome: R-HSA-449836Reactome: R-HSA-5250968Reactome: R-HSA-5250971Reactome: R-HSA-5250992Reactome: R-HSA-6798695Reactome: R-HSA-888590Reactome: R-HSA-9662360 |
| Cellular Localisation | CytoplasmPerinuclear RegionCell MembraneLipid-AnchorSynapseSynaptosomePhotoreceptor Inner SegmentMembrane Association Requires PalmitoylationExpressed Throughout CytoplasmConcentrating At The Perinuclear RegionColocalizes With Kcnb1 At The Cell MembraneColocalizes With Plcl1 At The Cell Membrane |
| Alternative Antibody Names | Anti-Synaptosomal-Associated Protein 25 antibodyAnti-Snap-25 antibodyAnti-Super Protein antibodyAnti-Sup antibodyAnti-Synaptosomal-Associated 25 Kda Protein antibodyAnti-SNAP25 antibodyAnti-SNAP antibody |
Information sourced from Uniprot.org