• Western blot analysis of lysates from Raw264.7 cells, treated with EGF 200ng/ml 30', using SNAP25 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SNAP25 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-SNAP25 antibody (151-200 aa) (STJ95717)

SKU:
STJ95717

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Synaptosomal-associated protein 25 (151-200 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNAP25
Gene ID: 6616
Uniprot ID: SNP25_HUMAN
Immunogen Region: 151-200 aa
Specificity: SNAP 25 Polyclonal Antibody detects endogenous levels of SNAP 25 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SNAP25 at the amino acid range 151-200
Post Translational Modifications Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release. C.botulinum type C only rarely infects humans. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release.
Function t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Protein Name Synaptosomal-Associated Protein 25
Snap-25
Super Protein
Sup
Synaptosomal-Associated 25 Kda Protein
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Reactome: R-HSA-422356
Reactome: R-HSA-449836
Reactome: R-HSA-5250968
Reactome: R-HSA-5250971
Reactome: R-HSA-5250992
Reactome: R-HSA-6798695
Reactome: R-HSA-888590
Reactome: R-HSA-9662360
Cellular Localisation Cytoplasm
Perinuclear Region
Cell Membrane
Lipid-Anchor
Synapse
Synaptosome
Photoreceptor Inner Segment
Membrane Association Requires Palmitoylation
Expressed Throughout Cytoplasm
Concentrating At The Perinuclear Region
Colocalizes With Kcnb1 At The Cell Membrane
Colocalizes With Plcl1 At The Cell Membrane
Alternative Antibody Names Anti-Synaptosomal-Associated Protein 25 antibody
Anti-Snap-25 antibody
Anti-Super Protein antibody
Anti-Sup antibody
Anti-Synaptosomal-Associated 25 Kda Protein antibody
Anti-SNAP25 antibody
Anti-SNAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance