• STJ71940 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71940 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-SMC5 antibody (Internal) (STJ71940)

SKU:
STJ71940-100

£312.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Mouse/Rat/Cow/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SMC5 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Preliminary testing showed bands at approx 100+150kDa in HEK293 nuclear lysates after 1µg/ml antibody staining (calculated MW of 129kDa according to NP_055925.2). These bands were suc
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SMC5
Gene ID: 23137
Uniprot ID: SMC5_HUMAN
Immunogen Region: Internal
Accession Number: NP_055925.2
Immunogen Sequence: TSKHLEHKDNELRQ
Post Translational Modifications Sumoylated. Ubiquitinated. (Microbial infection) SMC5-SMC6 complex is degraded by the activity of Hepatitis B X protein.
Function Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression.the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome.
Protein Name Structural Maintenance Of Chromosomes Protein 5
Smc Protein 5
Smc-5
Hsmc5
Database Links Reactome: R-HSA-3108214
Cellular Localisation Nucleus
Chromosome
Pml Body
Telomere
Associates With Chromatin
Colocalizes With Smc6 On The X-Y Chromosome Pair Within The Sex Vesicle During Late Pachytene/Diplotene
Localizes To Pml Nuclear Bodies In Alt Cell Lines
Accumulates With Rad18 And The Slf1-Slf2 Complex At Replication-Coupled Dna Interstrand Repair And Dna Double-Strand Breaks (Dsbs) Sites On Chromatin In A Ubiquitin-Dependent Manner
Alternative Antibody Names Anti-Structural Maintenance Of Chromosomes Protein 5 antibody
Anti-Smc Protein 5 antibody
Anti-Smc-5 antibody
Anti-Hsmc5 antibody
Anti-SMC5 antibody
Anti-KIAA0594 antibody
Anti-SMC5L1 antibody

Information sourced from Uniprot.org

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