• Western blot analysis of Rat thymus, using SMARCD2 antibody (STJ113998) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of mouse thymus, using SMARCD2 antibody (STJ113998) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SMARCD2 antibody (STJ113998) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMARCD2 antibody (454-531) (STJ113998)

SKU:
STJ113998

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMARCD2
Gene ID: 6603
Uniprot ID: SMRD2_HUMAN
Immunogen Region: 454-531
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 454-531 of human SMARCD2 (NP_001091896.1).
Immunogen Sequence: RDFMLSFSTDPQDFIQEWLR SQRRDLKIITDVIGNPEEER RAAFYHQPWAQEAVGRHIFA KVQQRRQELEQVLGIRLT
Tissue Specificity Isoform 2 is expressed in the pancreas.
Post Translational Modifications Ubiquitinated through a signaling process involving RAC1 and the RING finger protein UNKL.
Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation.
Protein Name Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily D Member 2
60 Kda Brg-1/Brm-Associated Factor Subunit B
Brg1-Associated Factor 60b
Baf60b
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8939243
Reactome: R-HSA-9824585
Reactome: R-HSA-9845323
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily D Member 2 antibody
Anti-60 Kda Brg-1/Brm-Associated Factor Subunit B antibody
Anti-Brg1-Associated Factor 60b antibody
Anti-Baf60b antibody
Anti-SMARCD2 antibody
Anti-BAF60B antibody
Anti-PRO2451 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance