| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-SLC9A1 (Cytoplasmic Domain) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | SLC9A1 |
| Gene ID: | 6548 |
| Uniprot ID: | SL9A1_HUMAN |
| Immunogen Region: | Cytoplasmic Domain |
| Specificity: | Specific for SL9A1. |
| Immunogen: | A synthetic peptide from cytoplasmic domain of Sodium/Hydrogen Exchanger 1 (SLC9A1, NHE1) conjugated to an immunogenic carrier protein was used as the antigen. |
| Post Translational Modifications | O-glycosylated. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1. Phosphorylation at Thr-779 increases SLC9A1 activity. Specifically dephosphorylated at Thr-779 by PPP3CA that negatively regulates SLC9A1 activity. Phosphorylation at Ser-648 by AKT1 reduces SLC9A1 binding to CALM1. Palmitoylated.may play a major role in SLC9A1 regulation. |
| Function | Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism. Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry. Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs. |
| Protein Name | Sodium/Hydrogen Exchanger 1 Apnh Na(+/H(+ Antiporter - Amiloride-Sensitive Na(+/H(+ Exchanger 1 Nhe-1 Solute Carrier Family 9 Member 1 |
| Database Links | Reactome: R-HSA-2160916 Reactome: R-HSA-425986 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Localized Basolaterally In Every Epithelial Cell Except In The Choroid Plexus Where Slc9a1 Is Expressed Luminally |
| Alternative Antibody Names | Anti-Sodium/Hydrogen Exchanger 1 antibody Anti-Apnh antibody Anti-Na(+/H(+ Antiporter - Amiloride-Sensitive antibody Anti-Na(+/H(+ Exchanger 1 antibody Anti-Nhe-1 antibody Anti-Solute Carrier Family 9 Member 1 antibody Anti-SLC9A1 antibody Anti-APNH1 antibody Anti-NHE1 antibody |
Information sourced from Uniprot.org