• Western blot analysis of extracts of various cell lines, using Glyt2 antibody (STJ11104119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SLC6A5 antibody (250-350) [S4119RM] (STJ11104119)

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STJ11104119

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Sodium-And Chloride-Dependent Glycine Transporter 2 (250-350) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4119RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC6A5
Gene ID: 9152
Uniprot ID: SC6A5_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Glyt2 (Q9Y345).
Immunogen Sequence: SLGQFASQGPVSVWKAIPAL QGCGIAMLIISVLIAIYYNV IICYTLFYLFASFVSVLPWG SCNNPWNTPECKDKTKLLLD SCVISDHPKIQIKNSTFCMT A
Tissue Specificity Expressed in medulla, and to a lesser extent in spinal cord and cerebellum.
Post Translational Modifications N-glycosylated.
Function Sodium- and chloride-dependent glycine transporter. Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses. Isoform 2: Lacks sodium- and chloride-dependent glycine transporter activity. Isoform 3: Lacks sodium- and chloride-dependent glycine transporter activity.
Protein Name Sodium- And Chloride-Dependent Glycine Transporter 2
Glyt-2
Glyt2
Solute Carrier Family 6 Member 5
Database Links Reactome: R-HSA-442660
Reactome: R-HSA-5619089
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Sodium- And Chloride-Dependent Glycine Transporter 2 antibody
Anti-Glyt-2 antibody
Anti-Glyt2 antibody
Anti-Solute Carrier Family 6 Member 5 antibody
Anti-SLC6A5 antibody
Anti-GLYT2 antibody
Anti-NET1 antibody

Information sourced from Uniprot.org

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