Western blot analysis of extracts of various cell lines, using SLC6A2 antibody (STJ29850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SLC6A2 antibody (30-235) (STJ29850)

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STJ29850
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC6A2
Gene ID: 6530
Uniprot ID: SC6A2_HUMAN
Immunogen Region: 30-235
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 30-235 of human SLC6A2 (NP_478066.3).
Immunogen Sequence: ELIPDAPLSSAAYSIRSIGE RPVLKAPVPKRQKCDHWTPC PSDTYAYRLLSGGGRSKYAK ICFEDNLLMGEQLGNVARGI NIAIVNYVTGNVTATRCFDM YEGDNSGPMTKFIQSAAPKS LLFMVTYDDGSTRLNNDAKN AIEALGSKEIRNMKFRSSWV FIAAKGLELPSEIQREKINH SDAKNNRYSGWPAEIQIEGC IPKERS
Post Translational Modifications Palmitoylated.palmitoylation regulates protein levels and neurotransmitter transport.
Function Mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline), the primary signaling neurotransmitter in the autonomic sympathetic nervous system. Is responsible for norepinephrine re-uptake and clearance from the synaptic cleft, thus playing a crucial role in norepinephrine inactivation and homeostasis. Can also mediate sodium- and chloride-dependent transport of dopamine.
Protein Name Sodium-Dependent Noradrenaline Transporter
Norepinephrine Transporter
Net
Solute Carrier Family 6 Member 2
Database Links Reactome: R-HSA-442660
Reactome: R-HSA-5619109
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Axon
Synapse
Synaptosome
Alternative Antibody Names Anti-Sodium-Dependent Noradrenaline Transporter antibody
Anti-Norepinephrine Transporter antibody
Anti-Net antibody
Anti-Solute Carrier Family 6 Member 2 antibody
Anti-SLC6A2 antibody
Anti-NAT1 antibody
Anti-NET1 antibody
Anti-SLC6A5 antibody

Information sourced from Uniprot.org

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