• Western blot analysis of extracts of various cell lines, using GLUT2/GLUT2/SLC2A2 antibody (STJ111885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of paraffin-embedded Human colon cancer using GLUT2/SLC2A2 Rabbit polyclonal antibody (STJ111885) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates, using GLUT2/SLC2A2 Rabbit polyclonal antibody (STJ111885) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SLC2A2 antibody (59-75) (STJ111885)

SKU:
STJ111885

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC2A2 (59-75) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC2A2
Gene ID: 6514
Uniprot ID: GTR2_HUMAN
Immunogen Region: 59-75
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 59-75 of human GLUT2/GLUT2/SLC2A2 (NP_000331.1).
Immunogen Sequence: YVINSTDELPTISYSMN
Tissue Specificity Liver, insulin-producing beta cell, small intestine and kidney.
Post Translational Modifications N-glycosylated.required for stability and retention at the cell surface of pancreatic beta cells.
Function Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose. Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells.may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Also able to mediate the transport of dehydroascorbate.
Protein Name Solute Carrier Family 2 - Facilitated Glucose Transporter Member 2
Glucose Transporter Type 2 - Liver
Glut-2
Database Links Reactome: R-HSA-189200
Reactome: R-HSA-210745
Reactome: R-HSA-422356
Reactome: R-HSA-5619098
Reactome: R-HSA-8981373
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Solute Carrier Family 2 - Facilitated Glucose Transporter Member 2 antibody
Anti-Glucose Transporter Type 2 - Liver antibody
Anti-Glut-2 antibody
Anti-SLC2A2 antibody
Anti-GLUT2 antibody

Information sourced from Uniprot.org

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