• Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using MCT4/SLC16A3 Rabbit polyclonal antibody (STJ112566) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using MCT4/SLC16A3 Rabbit polyclonal antibody (STJ112566) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MCT4/SLC16A3 antibody (STJ112566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SLC16A3 antibody (410-465) (STJ112566)

SKU:
STJ112566

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCT4/SLC16A3 (410-465) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC16A3
Gene ID: 9123
Uniprot ID: MOT4_HUMAN
Immunogen Region: 410-465
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 410-465 of human MCT4/SLC16A3 (NP_004198.1).
Immunogen Sequence: IRKKPKEPQPEVAAAEEEKL HKPPADSGVDLREVEHFLKA EPEKNGEVVHTPETSV
Tissue Specificity Highly expressed in skeletal muscle.
Function Proton-dependent transporter of monocarboxylates such as L-lactate and pyruvate. Plays a predominant role in L-lactate efflux from highly glycolytic cells.
Protein Name Monocarboxylate Transporter 4
Mct 4
Solute Carrier Family 16 Member 3
Database Links Reactome: R-HSA-210991
Reactome: R-HSA-433692
Reactome: R-HSA-70268
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Plasma Membrane Localization Is Dependent Upon The Bsg/Mct4 Interaction
Basolateral Sorting Signals (Blss) In C-Terminal Cytoplasmic Tail Ensure Its Basolateral Expression In Polarised Epithelial Cells
Alternative Antibody Names Anti-Monocarboxylate Transporter 4 antibody
Anti-Mct 4 antibody
Anti-Solute Carrier Family 16 Member 3 antibody
Anti-SLC16A3 antibody
Anti-MCT3 antibody
Anti-MCT4 antibody

Information sourced from Uniprot.org

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