• Immunofluorescence analysis of mouse kidney using SLC14A1 antibody (STJ118436) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using SLC14A1 Rabbit polyclonal antibody (STJ118436) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using SLC14A1 Rabbit polyclonal antibody (STJ118436) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SLC14A1 Rabbit polyclonal antibody (STJ118436) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of RT4, using SLC14A1 antibody (STJ118436) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SLC14A1 antibody (244-257) (STJ118436)

SKU:
STJ118436

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC14A1 (244-257) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC14A1
Gene ID: 6563
Uniprot ID: UT1_HUMAN
Immunogen Region: 244-257
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 244-257 of human SLC14A1 (NP_001295208.1).
Immunogen Sequence: FYLQAKKRMVESPL
Tissue Specificity Detected in erythrocytes (at protein level). Expressed in spleen erythroblasts and tumoral kidney.
Function Mediates the transport of urea driven by a concentration gradient across the cell membrane of erythrocytes. Also mediates the transport of urea across the cell membrane of the renal inner medullary collecting duct which is critical to the urinary concentrating mechanism. Facilitates water transport in erythrocytes.
Protein Name Urea Transporter 1
Solute Carrier Family 14 Member 1
Urea Transporter - Erythrocyte
Database Links Reactome: R-HSA-425366
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Restricted To The Basolateral Membrane In Various Portions Of The Urothelium
Alternative Antibody Names Anti-Urea Transporter 1 antibody
Anti-Solute Carrier Family 14 Member 1 antibody
Anti-Urea Transporter - Erythrocyte antibody
Anti-SLC14A1 antibody
Anti-HUT11 antibody
Anti-JK antibody
Anti-RACH1 antibody
Anti-UT1 antibody
Anti-UTE antibody

Information sourced from Uniprot.org

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