Host: | Goat |
Applications: | Pep-ELISA/IHC/IF/FC |
Reactivity: | Human/Mouse/Rat/Dog/Cow |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-SLC12A2 (Internal) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 3.75µg/ml.IF: Strong expression of the protein seen in the cytoplasm/vesicles of HeLa cells |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | SLC12A2 |
Gene ID: | 6558 |
Uniprot ID: | S12A2_HUMAN |
Immunogen Region: | Internal |
Accession Number: | NP_001037.1; NP_001243390.1 |
Immunogen Sequence: | NALQHSIRLSGVEDH |
Post Translational Modifications | Phosphorylated at Thr-203, Thr-207 and Thr-212 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity. |
Function | Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume. |
Protein Name | Solute Carrier Family 12 Member 2Basolateral Na-K-Cl SymporterBumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2Bsc2Na-K-2cl Cotransporter 1Hnkcc1 |
Database Links | Reactome: R-HSA-426117 |
Cellular Localisation | Basolateral Cell MembraneMulti-Pass Membrane Protein |
Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 2 antibodyAnti-Basolateral Na-K-Cl Symporter antibodyAnti-Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2 antibodyAnti-Bsc2 antibodyAnti-Na-K-2cl Cotransporter 1 antibodyAnti-Hnkcc1 antibodyAnti-SLC12A2 antibodyAnti-NKCC1 antibody |
Information sourced from Uniprot.org