| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC/IF/ICC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-SLC12A1/NKCC (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.WB: This anitbody has been successfully used in WB on Mouse: Alshahrani S et al. (2015) PMID: 26400961.IHC: In paraffin embedded Human Kidney shows strong membrane staining in some CT |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
SLC12A1 |
| Gene ID: |
6557 |
| Uniprot ID: |
S12A1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_000329.2 |
| Immunogen Sequence: |
PHYEETSFGDEAQKR |
| Post Translational Modifications | Phosphorylated at Ser-91, Thr-100 and Thr-105 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity. |
| Function | Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation. Electrically silent transporter system. |
| Protein Name | Solute Carrier Family 12 Member 1Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 1Bsc1Kidney-Specific Na-K-Cl SymporterNa-K-2cl Cotransporter 2Nkcc2 |
| Database Links | Reactome: R-HSA-426117Reactome: R-HSA-5619104 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 1 antibodyAnti-Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 1 antibodyAnti-Bsc1 antibodyAnti-Kidney-Specific Na-K-Cl Symporter antibodyAnti-Na-K-2cl Cotransporter 2 antibodyAnti-Nkcc2 antibodyAnti-SLC12A1 antibodyAnti-NKCC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
