• Western blot analysis of extracts of various cell lines, using SLC12A1/NKCC2 antibody (STJ11104121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SLC12A1 antibody (601-800) [S4121RM] (STJ11104121)

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STJ11104121

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Solute Carrier Family 12 Member 1 (601-800) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4121RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC12A1
Gene ID: 6557
Uniprot ID: S12A1_HUMAN
Immunogen Region: 601-800
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 601-800 of human SLC12A1/NKCC2 (Q13621).
Immunogen Sequence: YGIYNMWVSLFGAVLCCAVM FVINWWAAVITYVIEFFLYV YVTCKKPDVNWGSSTQALSY VSALDNALELTTVEDHVKNF RPQCIVLTGGPMTRPALLDI THAFTKNSGLCICCEVFVGP RKLCVKEMNSGMAKKQAWLI KNKIKAFYAAVAADCFRDGV RSLLQASGLGRMKPNTLVIG YKKNWRKAPLTEIENYVGII
Tissue Specificity Kidney.localizes to the thick ascending limbs (at protein level).
Post Translational Modifications Phosphorylated at Ser-91, Thr-100 and Thr-105 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity.
Function Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation. Electrically silent transporter system.
Protein Name Solute Carrier Family 12 Member 1
Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2
Kidney-Specific Na-K-Cl Symporter
Database Links Reactome: R-HSA-426117
Reactome: R-HSA-5619104
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Solute Carrier Family 12 Member 1 antibody
Anti-Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2 antibody
Anti-Kidney-Specific Na-K-Cl Symporter antibody
Anti-SLC12A1 antibody
Anti-NKCC2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance