• Western blot analysis of lysates from 293 cells, using SKP2/p45 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of A549 cells, using SKP2/p45 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using SKP2/p45 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of various cells using Skp2 p45 Polyclonal Antibody diluted at 1：500
  • Immunofluorescence analysis of Hela cell. 1, Skp2 p45 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SKP2 antibody (366-415 aa) (STJ95676)

SKU:
STJ95676

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-S-phase kinase-associated protein 2 (366-415 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SKP2
Gene ID: 6502
Uniprot ID: SKP2_HUMAN
Immunogen Region: 366-415 aa
Specificity: Skp2 p45 Polyclonal Antibody detects endogenous levels of Skp2 p45 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SKP2/p45 at the amino acid range 366-415
Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.
Protein Name S-Phase Kinase-Associated Protein 2
Cyclin-A/Cdk2-Associated Protein P45
F-Box Protein Skp2
F-Box/Lrr-Repeat Protein 1
P45skp2
Database Links Reactome: R-HSA-174178
Reactome: R-HSA-187577
Reactome: R-HSA-5689880
Reactome: R-HSA-68949
Reactome: R-HSA-69231
Reactome: R-HSA-8939902
Reactome: R-HSA-8951664
Reactome: R-HSA-9687136
Reactome: R-HSA-9708530
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-S-Phase Kinase-Associated Protein 2 antibody
Anti-Cyclin-A/Cdk2-Associated Protein P45 antibody
Anti-F-Box Protein Skp2 antibody
Anti-F-Box/Lrr-Repeat Protein 1 antibody
Anti-P45skp2 antibody
Anti-SKP2 antibody
Anti-FBXL1 antibody

Information sourced from Uniprot.org

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