| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SKAP1 |
| Gene ID: | 8631 |
| Uniprot ID: | SKAP1_HUMAN |
| Immunogen Region: | 1-358 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-358 of human SKAP1 (NP_001068567.1). |
| Immunogen Sequence: | MQAAALPEEIRWLLEDAEEF LAEGLRNENLSAVARDHRDH ILRGFQQIKARYYWDFQPQG GDIGQDSSDDNHSGTLGLSL TSDAPFLSDYQDEGMEDIVK GAQELDNVIKQGYLEKKSKD HSFFGSEWQKRWCVVSRGLF YYYANEKSKQPKGTFLIKGY GVRMAPHLRRDSKKESCFEL TSQDRRSYEFTATSPAEARD WVDQISFLLKDLSSLTIPYE EDEEEEEKEETYDDIDGFD |
| Tissue Specificity | Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level). |
| Post Translational Modifications | Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB1. Tyr-232 is dephosphorylated by PTPRC (Probable). |
| Function | Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. |
| Protein Name | Src Kinase-Associated Phosphoprotein 1 Src Family-Associated Phosphoprotein 1 Src Kinase-Associated Phosphoprotein Of 55 Kda Skap-55 Pp55 |
| Cellular Localisation | Cytoplasm Nucleus Cell Membrane Upon T-Cell Stimulation Translocates To Lipid Rafts At The Cell Membrane |
| Alternative Antibody Names | Anti-Src Kinase-Associated Phosphoprotein 1 antibody Anti-Src Family-Associated Phosphoprotein 1 antibody Anti-Src Kinase-Associated Phosphoprotein Of 55 Kda antibody Anti-Skap-55 antibody Anti-Pp55 antibody Anti-SKAP1 antibody Anti-SCAP1 antibody Anti-SKAP55 antibody |
Information sourced from Uniprot.org