Anti-SIN3B antibody (221-270 aa) (STJ94263)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human SIN3B at the amino acid range 221-270
General Information
| Short Description | Rabbit polyclonal anti-Paired amphipathic helix protein Sin3b (221-270 aa) for use in WB, ELISA and IHC in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SIN3B |
| Gene ID | 23309 |
| Uniprot ID | SIN3B_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human SIN3B at the amino acid range 221-270 |
| Immunogen Region | 221-270 aa |
| Specificity | Sin3B Polyclonal Antibody detects endogenous levels of Sin3B protein. |
Additional Info
| Function | Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression. As part of the SIN3B complex represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2. SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription. |
| Protein Name | Paired Amphipathic Helix Protein Sin3bHistone Deacetylase Complex Subunit Sin3bTranscriptional Corepressor Sin3b |
| Database Links | Reactome: R-HSA-400206 O75182-1Reactome: R-HSA-427413Reactome: R-HSA-8936459Reactome: R-HSA-9707564 O75182-1 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Paired Amphipathic Helix Protein Sin3b antibodyAnti-Histone Deacetylase Complex Subunit Sin3b antibodyAnti-Transcriptional Corepressor Sin3b antibodyAnti-SIN3B antibodyAnti-KIAA0700 antibody |
Information sourced from Uniprot.org