• Western blot analysis of lysates from U-937 cells, using SIGLEC6 Rabbit polyclonal antibody (STJ114265) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SIGLEC6 antibody (27-220) (STJ114265)

SKU:
STJ114265

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIGLEC6 (27-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIGLEC6
Gene ID: 946
Uniprot ID: SIGL6_HUMAN
Immunogen Region: 27-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-220 of human SIGLEC6 (NP_942142.3).
Immunogen Sequence: QERRFQLEGPESLTVQEGLC VLVPCRLPTTLPASYYGYGY WFLEGADVPVATNDPDEEVQ EETRGRFHLLWDPRRKNCSL SIRDARRRDNAAYFFRLKSK WMKYGYTSSKLSVRVMALTH RPNISIPGTLESGHPSNLTC SVPWVCEQGTPPIFSWMSAA PTSLGPRTTQSSVLTITPRP QDHSTNLTCQVTFP
Tissue Specificity Expressed at high levels in placenta (cyto- and syncytiotrophoblastic cells) and at lower levels in spleen, peripheral blood leukocytes (predominantly B-cells) and small intestine.
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Protein Name Sialic Acid-Binding Ig-Like Lectin 6
Siglec-6
Cd33 Antigen-Like 1
Cdw327
Obesity-Binding Protein 1
Ob-Bp1
Cd Antigen Cd327
Database Links Reactome: R-HSA-198933
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Sialic Acid-Binding Ig-Like Lectin 6 antibody
Anti-Siglec-6 antibody
Anti-Cd33 Antigen-Like 1 antibody
Anti-Cdw327 antibody
Anti-Obesity-Binding Protein 1 antibody
Anti-Ob-Bp1 antibody
Anti-Cd Antigen Cd327 antibody
Anti-SIGLEC6 antibody
Anti-CD33L antibody
Anti-CD33L1 antibody
Anti-OBBP1 antibody

Information sourced from Uniprot.org

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