• Western blot analysis of various lysates using SIAE Rabbit polyclonal antibody (STJ115183) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SIAE antibody (270-450) (STJ115183)

SKU:
STJ115183

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIAE
Gene ID: 54414
Uniprot ID: SIAE_HUMAN
Immunogen Region: 270-450
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 270-450 of human SIAE (NP_733746.1).
Immunogen Sequence: LKGVVWYQGESNINYNTDLY NCTFPALIEDWRETFHRGSQ GQTERFFPFGLVQLSSDLSK KSSDDGFPQIRWHQTADFGY VPNPKMPNTFMAVAMDLCDR DSPFGSIHPRDKQTVAYRLH LGARALAYGEKNLTFEGPLP EKIELLAHKGLLNLTYYQQI QVQKKDNKIFEISCCSDHRC K
Tissue Specificity Widely expressed with high expression in the testis, prostate, and colon.
Function Catalyzes the removal of O-acetyl ester groups from position 9 of the free diacetylated sialate N-acetyl-9-O-acetylneuraminate (Neu5,9Ac2) in the cytosol and of the diacetylated sialate residues of sialylglycoconjugates in the lysosomes (Probable). Together with the sialate-O-acetyltransferase they regulate the balance of acetylated sialoglycoconjugates, key players in various processes such as cell-cell interactions, host-pathogen recognition, and tumor antigenicity.
Protein Name Sialate O-Acetylesterase
Siae
H-Lse
Sialic Acid-Specific 9-O-Acetylesterase
Cellular Localisation Isoform 1: Lysosome
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Sialate O-Acetylesterase antibody
Anti-Siae antibody
Anti-H-Lse antibody
Anti-Sialic Acid-Specific 9-O-Acetylesterase antibody
Anti-SIAE antibody
Anti-YSG2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance