Western blot analysis of various lysates, using SGPL1 rabbit polyclonal antibody (STJ118190) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SGPL1 antibody (60-390) (STJ118190)

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STJ118190
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SGPL1
Gene ID: 8879
Uniprot ID: SGPL1_HUMAN
Immunogen Region: 60-390
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 60-390 of human SGPL1 (NP_003892.2).
Immunogen Sequence: VFQPESLWSRFKKKCFKLTR KMPIIGRKIQDKLNKTKDDI SKNMSFLKVDKEYVKALPSQ GLSSSAVLEKLKEYSSMDAF WQEGRASGTVYSGEEKLTEL LVKAYGDFAWSNPLHPDIFP GLRKIEAEIVRIACSLFNGG PDSCGCVTSGGTESILMACK AYRDLAFEKGIKTPEIVAPQ SAHAAFNKAASYFGMKIVRV PLTKMMEVDVRAMRRAISRN TAMLVCSTPQFPHGVIDPV
Tissue Specificity Ubiquitously expressed. Expressed in fetal and adult adrenal gland (at protein level).
Function Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP. Seems to play a role in establishing neuronal contact sites and axonal maintenance.
Protein Name Sphingosine-1-Phosphate Lyase 1
S1pl
Sp-Lyase 1
Spl 1
Hspl
Sphingosine-1-Phosphate Aldolase
Database Links Reactome: R-HSA-9845614
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iii Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Sphingosine-1-Phosphate Lyase 1 antibody
Anti-S1pl antibody
Anti-Sp-Lyase 1 antibody
Anti-Spl 1 antibody
Anti-Hspl antibody
Anti-Sphingosine-1-Phosphate Aldolase antibody
Anti-SGPL1 antibody
Anti-KIAA1252 antibody

Information sourced from Uniprot.org

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