| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SGK3 (1-150) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SGK3 |
| Gene ID: |
100533105/23678 |
| Uniprot ID: |
SGK3_HUMAN |
| Immunogen Region: |
1-150 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SGK3 (NP_037389.4). |
| Immunogen Sequence: |
MQRDHTMDYKESCPSVSIPS SDEHREKKKRFTVYKVLVSV GRSEWFVFRRYAEFDKLYNT LKKQFPAMALKIPAKRIFGD NFDPDFIKQRRAGLNEFIQN LVRYPELYNHPDVRAFLQMD SPKHQSDPSEDEDERSSQKL HSTSQNINLG |
| Tissue Specificity | Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER-positive breast tumors than ER-negative breast tumors. |
| Post Translational Modifications | Activated by phosphorylation on Ser-486 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320. |
| Function | Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes. |
| Protein Name | Serine/Threonine-Protein Kinase Sgk3Cytokine-Independent Survival KinaseSerum/Glucocorticoid-Regulated Kinase 3Serum/Glucocorticoid-Regulated Kinase-Like |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cytoplasmic VesicleEarly EndosomeRecycling EndosomeEndosomal Localization Is A Prerequisite For Complete Kinase ActivityIt Is Essential For Its Colocalization With The Kinase Responsible For Phosphorylating Ser-486 Thus Allowing Pdpk1 Phosphorylation Of Thr-320 Resulting In Complete Activation Of Sgk3Localized In Vesicle-Like Structures And In The Early EndosomeColocalizes With Slc9a3/Nhe3 In The Recycling Endosomes |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Sgk3 antibodyAnti-Cytokine-Independent Survival Kinase antibodyAnti-Serum/Glucocorticoid-Regulated Kinase 3 antibodyAnti-Serum/Glucocorticoid-Regulated Kinase-Like antibodyAnti-SGK3 antibodyAnti-CISK antibodyAnti-SGKL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
