| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SFXN1 |
| Gene ID: |
94081 |
| Uniprot ID: |
SFXN1_HUMAN |
| Immunogen Region: |
87-322 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 87-322 of human SFXN1 (NP_073591.2). |
| Immunogen Sequence: |
MILIGRMSAQVPMNMTITGC MMTFYRTTPAVLFWQWINQS FNAVVNYTNRSGDAPLTVNE LGTAYVSATTGAVATALGLN ALTKHVSPLIGRFVPFAAVA AANCINIPLMRQRELKVGIP VTDENGNRLGESANAAKQAI TQVVVSRILMAAPGMAIPPF IMNTLEKKAFLKRFPWMSAP IQVGLVGFCLVFATPLCCAL FPQKSSMSVTSLEAELQAKI QESHPELRRVYFNKGL |
| Tissue Specificity | Highly expressed in tissues with high one-carbon metabolism activity, such as blood, liver and kidney. |
| Function | Amino acid transporter importing serine, an essential substrate of the mitochondrial branch of the one-carbon pathway, into mitochondria. Mitochondrial serine is then converted to glycine and formate, which exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors. May also transport other amino acids including alanine and cysteine. |
| Protein Name | Sideroflexin-1 |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Sideroflexin-1 antibodyAnti-SFXN1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SPK knockout (KO) 293T cells, using [KO Validated] SPK Rabbit polyclonal antibody (STJ11100823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89759/358235/STJ11100823_1__81500.1713122567.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SPK knockout (KO) 293T cells, using [KO Validated] SPK Rabbit polyclonal antibody (STJ11100823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
