| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SF3B1 (1-144) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SF3B1 |
| Gene ID: |
23451 |
| Uniprot ID: |
SF3B1_HUMAN |
| Immunogen Region: |
1-144 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1). |
| Immunogen Sequence: |
MAKIAKTHEDIEAQIREIQG KKAALDEAQGVGLDSTGYYD QEIYGGSDSRFAGYVTSIAA TELEDDDDDYSSSTSLLGQK KPGYHAPVALLNDIPQSTEQ YDPFAEHRPPKIADREDEYK KHRRTMIISPERLDPFADGF YSAA |
| Post Translational Modifications | Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis. Citrullinated by PADI4. |
| Function | Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92. May also be involved in the assembly of the 'E' complex. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). |
| Protein Name | Splicing Factor 3b Subunit 1Pre-Mrna-Splicing Factor Sf3b 155 Kda SubunitSf3b155Spliceosome-Associated Protein 155Sap 155 |
| Database Links | Reactome: R-HSA-5250924Reactome: R-HSA-72163Reactome: R-HSA-72165 |
| Cellular Localisation | NucleusNucleus SpeckleDuring MitosisTransiently Dispersed From The Nuclear Speckles To The Cytoplasm |
| Alternative Antibody Names | Anti-Splicing Factor 3b Subunit 1 antibodyAnti-Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit antibodyAnti-Sf3b155 antibodyAnti-Spliceosome-Associated Protein 155 antibodyAnti-Sap 155 antibodyAnti-SF3B1 antibodyAnti-SAP155 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
