• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SETMAR antibody (350-400 aa) (STJ95625)

SKU:
STJ95625

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone-lysine N-methyltransferase SETMAR (350-400 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SETMAR
Gene ID: 6419
Uniprot ID: SETMR_HUMAN
Immunogen Region: 350-400 aa
Specificity: SETMAR Polyclonal Antibody detects endogenous levels of SETMAR protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SETMAR at the amino acid range 350-400
Post Translational Modifications Methylated. Methylation regulates activity in DNA decatenation. Phosphorylated at Ser-508 by CHEK1 and dephosphorylated by protein phosphatase 2A/PP2A. Phosphorylation at Ser-508 is enhanced by DNA damage and promotes recruitment to damaged DNA. It stimulates DNA repair and impairs replication fork restart.
Function Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining. Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A.
Protein Name Histone-Lysine N-Methyltransferase Setmar
Set Domain And Mariner Transposase Fusion Protein
Metnase Includes - Histone-Lysine N-Methyltransferase - Transposon Hsmar1 Transposase
Cellular Localisation Nucleus
Chromosome
Recruited On Damaged Dna At Sites Of Double-Strand Breaks
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Setmar antibody
Anti-Set Domain And Mariner Transposase Fusion Protein antibody
Anti-Metnase Includes - Histone-Lysine N-Methyltransferase - Transposon Hsmar1 Transposase antibody
Anti-SETMAR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance