• Immunohistochemistry analysis of paraffin-embedded mouse brain using SETD5 Rabbit polyclonal antibody (STJ29443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using SETD5 Rabbit polyclonal antibody (STJ29443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SETD5 antibody (STJ29443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SETD5 antibody (1054-1153) (STJ29443)

SKU:
STJ29443

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SETD5 (1054-1153) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SETD5
Gene ID: 55209
Uniprot ID: SETD5_HUMAN
Immunogen Region: 1054-1153
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1054-1153 of human SETD5 (NP_001073986.1).
Immunogen Sequence: PSAPQNPPQRKKVSLLEYRK RKQEAKENSAGGGGDSAQSK SKSAGAGQGSSNSVSDTGAH GVQGSSARTPSSPHKKFSPS HSSMSHLEAVSPSDSRGTSS
Function Chromatin regulator required for brain development: acts as a regulator of RNA elongation rate, thereby regulating neural stem cell (NSC) proliferation and synaptic transmission. May act by mediating trimethylation of 'Lys-36' of histone H3 (H3K36me3), which is essential to allow on-time RNA elongation dynamics. Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. The relevance of histone methyltransferase activity is however subject to discussion.
Protein Name Histone-Lysine N-Methyltransferase Setd5
Set Domain-Containing Protein 5
Cellular Localisation Nucleus
Chromosome
Localizes To Active Transcribed Genes
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Setd5 antibody
Anti-Set Domain-Containing Protein 5 antibody
Anti-SETD5 antibody
Anti-KIAA1757 antibody

Information sourced from Uniprot.org

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