| Short Description : | Rabbit polyclonal Actin-Histidine N-Methyltransferase (72-329) antibody for use in WB, IP and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SETD3 |
| Gene ID: | 84193 |
| Uniprot ID: | SETD3_HUMAN |
| Immunogen Region: | 72-329 |
| Immunogen Sequence: | DGKREDYFPDLMKWASENGA SVEGFEMVNFKEEGFGLRAT RDIKAEELFLWVPRKLLMTV ESAKNSVLGPLYSQDRILQA MGNIALAFHLLCERASPNSF WQPYIQTLPSEYDTPLYFEE DEVRYLQSTQAIHDVFSQYK NTARQYAYFYKVIQTHPHAN KLPLKDSFTYEDYRWAVSSV MTRQNQIPTEDGSRVTLALI PLWDMCNHTNGLITTGYNLE DDRCECVALQDFRAGEQIY |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 72-329 of human SETD3 (NP_115609.2). |
| Post Translational Modifications | Phosphorylated by GSK3B, which is required for recognition by the SCF(FBXW7) complex and subsequent degradation. Ubiquitinated by the SCF(FBXW7) complex following phosphorylation by GSK3B, leading to its degradation by the proteasome. |
| Function | Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin. |
| Protein Name | Actin-Histidine N-MethyltransferaseProtein-L-Histidine N-Tele-MethyltransferaseSet Domain-Containing Protein 3Hsetd3 |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | CytoplasmNucleusLocalizes Mainly In The Cytoplasm |
| Alternative Antibody Names | Anti-Actin-Histidine N-Methyltransferase antibodyAnti-Protein-L-Histidine N-Tele-Methyltransferase antibodyAnti-Set Domain-Containing Protein 3 antibodyAnti-Hsetd3 antibodyAnti-SETD3 antibodyAnti-C14orf154 antibody |
Information sourced from Uniprot.org
