• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g SETD3 antibody (STJ11104476). Western blot was performed from the immunoprecipitate using SETD3 antibody (STJ11104476) at a dilution of 1:1000.
  • Western blot analysis of lysates from Jurkat cells, using SETD3 Rabbit polyclonal antibody (STJ11104476) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-SETD3 antibody (72-329) (STJ11104476)

SKU:
STJ11104476

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Actin-Histidine N-Methyltransferase (72-329) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SETD3
Gene ID: 84193
Uniprot ID: SETD3_HUMAN
Immunogen Region: 72-329
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 72-329 of human SETD3 (NP_115609.2).
Immunogen Sequence: DGKREDYFPDLMKWASENGA SVEGFEMVNFKEEGFGLRAT RDIKAEELFLWVPRKLLMTV ESAKNSVLGPLYSQDRILQA MGNIALAFHLLCERASPNSF WQPYIQTLPSEYDTPLYFEE DEVRYLQSTQAIHDVFSQYK NTARQYAYFYKVIQTHPHAN KLPLKDSFTYEDYRWAVSSV MTRQNQIPTEDGSRVTLALI PLWDMCNHTNGLITTGYNLE DDRCECVALQDFRAGEQIY
Post Translational Modifications Phosphorylated by GSK3B, which is required for recognition by the SCF(FBXW7) complex and subsequent degradation. Ubiquitinated by the SCF(FBXW7) complex following phosphorylation by GSK3B, leading to its degradation by the proteasome.
Function Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.
Protein Name Actin-Histidine N-Methyltransferase
Protein-L-Histidine N-Tele-Methyltransferase
Set Domain-Containing Protein 3
Hsetd3
Database Links Reactome: R-HSA-3214841
Cellular Localisation Cytoplasm
Nucleus
Localizes Mainly In The Cytoplasm
Alternative Antibody Names Anti-Actin-Histidine N-Methyltransferase antibody
Anti-Protein-L-Histidine N-Tele-Methyltransferase antibody
Anti-Set Domain-Containing Protein 3 antibody
Anti-Hsetd3 antibody
Anti-SETD3 antibody
Anti-C14orf154 antibody

Information sourced from Uniprot.org

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