| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SETD2 (1223-1430) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SETD2 |
| Gene ID: |
29072 |
| Uniprot ID: |
SETD2_HUMAN |
| Immunogen Region: |
1223-1430 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1223-1430 of human SETD2 (NP_054878.5). |
| Immunogen Sequence: |
QNRPDSRLGKTELSFSSSCE IPHVDGLHSSEELRNLGWDF SQEKPSTTYQQPDSSYGACG GHKYQQNAEQYGGTRDYWQG NGYWDPRSGRPPGTGVVYDR TQGQVPDSLTDDREEEENWD QQDGSHFSDQSDKFLLSLQK DKGSVQAPEISSNSIKDTLA VNEKKDFSKNLEKNDIKDRG PLKKRRQEIESDSESDGELQ DRKKVRVE |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | May be automethylated. |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. It is capable of trimethylating unmethylated H3K36 (H3K36me0) in vitro. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. Required for DNA double-strand break repair in response to DNA damage: acts by mediating formation of H3K36me3, promoting recruitment of RAD51 and DNA repair via homologous recombination (HR). Acts as a tumor suppressor. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Required for endoderm development by promoting embryonic stem cell differentiation toward endoderm: acts by mediating formation of H3K36me3 in distal promoter regions of FGFR3, leading to regulate transcription initiation of FGFR3. In addition to histones, also mediates methylation of other proteins, such as tubulins and STAT1. Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha-TubK40me3).alpha-TubK40me3 is required for normal mitosis and cytokinesis and may be a specific tag in cytoskeletal remodeling. Involved in interferon-alpha-induced antiviral defense by mediating both monomethylation of STAT1 at 'Lys-525' and catalyzing H3K36me3 on promoters of some interferon-stimulated genes (ISGs) to activate gene transcription. (Microbial infection) Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. |
| Protein Name | Histone-Lysine N-Methyltransferase Setd2Hif-1Huntingtin Yeast Partner BHuntingtin-Interacting Protein 1Hip-1Huntingtin-Interacting Protein BLysine N-Methyltransferase 3aProtein-Lysine N-Methyltransferase Setd2Set Domain-Containing Protein 2Hset2P231hbp |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Setd2 antibodyAnti-Hif-1 antibodyAnti-Huntingtin Yeast Partner B antibodyAnti-Huntingtin-Interacting Protein 1 antibodyAnti-Hip-1 antibodyAnti-Huntingtin-Interacting Protein B antibodyAnti-Lysine N-Methyltransferase 3a antibodyAnti-Protein-Lysine N-Methyltransferase Setd2 antibodyAnti-Set Domain-Containing Protein 2 antibodyAnti-Hset2 antibodyAnti-P231hbp antibodyAnti-SETD2 antibodyAnti-HIF1 antibodyAnti-HYPB antibodyAnti-KIAA1732 antibodyAnti-KMT3A antibodyAnti-SET2 antibodyAnti-HSPC069 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
