| Short Description : | Rabbit polyclonal PEDF (141-240) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SERPINF1 |
| Gene ID: | 5176 |
| Uniprot ID: | PEDF_HUMAN |
| Immunogen Region: | 141-240 |
| Immunogen Sequence: | RIVFEKKLRIKSSFVAPLEK SYGTRPRVLTGNPRLDLQEI NNWVQAQMKGKLARSTKEIP DEISILLLGVAHFKGQWVTK FDSRKTSLEDFYLDEERTVR |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 141-240 of human PEDF/SERPINF1 (NP_002606.3). |
| Tissue Specificity | Retinal pigment epithelial cells and blood plasma. |
| Post Translational Modifications | The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
| Function | Neurotrophic protein.induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
| Protein Name | Pigment Epithelium-Derived FactorPedfCell Proliferation-Inducing Gene 35 ProteinEpc-1Serpin F1 |
| Database Links | |
| Cellular Localisation | SecretedMelanosomeEnriched In Stage I Melanosomes |
| Alternative Antibody Names | Anti-Pigment Epithelium-Derived Factor antibodyAnti-Pedf antibodyAnti-Cell Proliferation-Inducing Gene 35 Protein antibodyAnti-Epc-1 antibodyAnti-Serpin F1 antibodyAnti-SERPINF1 antibodyAnti-PEDF antibodyAnti-PIG35 antibody |
Information sourced from Uniprot.org
