• Western blot analysis of extracts of Rat placenta, using PAI-1/Serpin E1 antibody (STJ27967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of PAI-1/Serpin E1 in paraffin-embedded human liver tissue using PAI-1/Serpin E1 rabbit polyclonal antibody (STJ27967) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PAI-1/Serpin E1 in paraffin-embedded mouse kidney tissue using PAI-1/Serpin E1 rabbit polyclonal antibody (STJ27967) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PAI-1/Serpin E1 in paraffin-embedded rat colon tissue using PAI-1/Serpin E1 rabbit polyclonal antibody (STJ27967) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of human liver cancer using PAI-1/Serpin E1 Polyclonal Antibody (STJ27967) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PAI-1/Serpin E1 antibody (STJ27967) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SERPINE1 antibody (24-199) (STJ27967)

SKU:
STJ27967

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SERPINE1 (24-199) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINE1
Gene ID: 5054
Uniprot ID: PAI1_HUMAN
Immunogen Region: 24-199
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 24-199 of human PAI-1/Serpin E1 (NP_000593.1).
Immunogen Sequence: AQASKDRNVVFSPYGVASVL AMLQLTTGGETQQQIQAAMG FKIDDKGMAPALRHLYKELM GPWNKDEISTTDAIFVQRDL KLVQGFMPHFFRLFRSTVKQ VDFSEVERARFIINDWVKTH TKGMISNLLGKGAVDQLTRL VLVNALYFNGQWKTPFPDSS THRRLFHKSDGSTVSV
Tissue Specificity Expressed in endothelial cells. Found in plasma, platelets, and hepatoma and fibrosarcoma cells.
Post Translational Modifications Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.
Function Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
Protein Name Plasminogen Activator Inhibitor 1
Pai
Pai-1
Endothelial Plasminogen Activator Inhibitor
Serpin E1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1368108
Reactome: R-HSA-2173796
Reactome: R-HSA-3000178
Reactome: R-HSA-75205
Cellular Localisation Secreted
Alternative Antibody Names Anti-Plasminogen Activator Inhibitor 1 antibody
Anti-Pai antibody
Anti-Pai-1 antibody
Anti-Endothelial Plasminogen Activator Inhibitor antibody
Anti-Serpin E1 antibody
Anti-SERPINE1 antibody
Anti-PAI1 antibody
Anti-PLANH1 antibody

Information sourced from Uniprot.org

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